Active Recombinant Human BRDT, His&FLAG-tagged

• Cat.No.: BRDT-37H
• Product Overview: Recombinant Human BRDT corresponding to amino acids 21-137 that contains the bromodomain sequences of BRDT (accession number NP_001717.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His
• Protein Length: 21-137 a.a.
• Description: BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a "reader" of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
• Form: 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
• Bio-activity: Binding specificity for acetylated H2BK12/15, H3K9/14/18/23, H4K12, H4K5/8, H4K8/12, H4K12/16, H4K5/12, H4K5/8/12/16 and H4K5/8/12/16.
• Molecular Mass: 20 kDa
• Applications: Binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 1.9 mg/ml

Gene Information

• Gene Name: BRDT bromodomain, testis-specific [ Homo sapiens ]
• Official Symbol: BRDT
• Synonyms: BRDT; bromodomain, testis-specific; bromodomain testis-specific protein; BRD6; cancer/testis antigen 9; CT9; RING3-like protein
• Gene ID: 676
• mRNA Refseq: NM_001242805
• Protein Refseq: NP_001229734
• MIM: 602144
• UniProt ID: Q58F21
• Chromosome Location: 1p22.1
• Function: histone binding; protein serine/threonine kinase activity; transcription coactivator activity