Active Recombinant Human BTK, His-tagged

• Cat.No.: IBTK-1370H
• Product Overview: Recombinant full-length human BTK was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: Full length
• Description: BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors. Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments.
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
• Bio-activity: 43 nmol/min/mg
• Molecular Mass: ~78 kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: IBTK inhibitor of Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Official Symbol: IBTK
• Synonyms: IBTK; inhibitor of Bruton agammaglobulinemia tyrosine kinase; Bruton agammaglobulinemia tyrosine kinase inhibitor , BTKI; inhibitor of Bruton tyrosine kinase; DKFZP564B116; BTK-binding protein; inhibitor of Brutons tyrosine kinase; inhibitor of Brutons tyrosine kinase-beta; inhibitor of Brutons tyrosine kinase-alpha; inhibitor of Brutons tyrosine kinase-gamma; Bruton agammaglobulinemia tyrosine kinase inhibitor; BTKI; RP1-93K22.1; KIAA1417; MGC142256; MGC142258
• Gene ID: 25998
• mRNA Refseq: NM_015525
• Protein Refseq: NP_056340
• MIM: 606457
• UniProt ID: Q9P2D0
• Chromosome Location: 6q14.3
• Pathway: BCR signaling pathway, organism-specific biosystem
• Function: protein kinase binding; protein tyrosine kinase inhibitor activity