| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc |
| Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human Complement C5a, Fc Tag at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 2-16 ng/mL. |
| Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Leu 679-Arg 751 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
