| Source : |
E.coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human Complement C5a, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-4 ng/ml. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 10.2 kDa. The protein migrates as 14-15 kDa under reducing (R) condition (SDS-PAGE). |
| Protein Length : |
Leu 679-Arg 751 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
