Active Recombinant Human CAMKK1 Protein, GST-Tagged

• Cat.No.: CAMKK1-0338H
• Product Overview: Human CAMKK1 (NP_115670.1, 1 a.a. - 505 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. cells.

Specification

• Description: The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Source: Insect Cell
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CAMKK substrate. ATP: 500 μM
• Molecular Mass: 82 kDa
• Purity: 72 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CAMKK1 calcium/calmodulin-dependent protein kinase kinase 1, alpha [ Homo sapiens ]
• Official Symbol: CAMKK1
• Synonyms: CAMKK1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; calcium/calmodulin-dependent protein kinase kinase 1; CAMKKA; DKFZp761M0423; MGC34095; caMKK 1; caM-KK 1; caM-KK alpha; CAMKK alpha protein; caM-kinase kinase 1; caM-kinase IV kinase; caM-kinase kinase alpha; calcium/calmodulin-dependent protein kinase kinase alpha
• Gene ID: 84254
• mRNA Refseq: NM_032294
• Protein Refseq: NP_115670
• MIM: 611411
• UniProt ID: Q8N5S9