Active Recombinant Human CASP3, Met&His-tagged

• Cat.No.: CASP3-192H
• Product Overview: Recombinant Human CASP3 (Accession # AAA65015) Met1-His277, fused with an N-terminal Met and 6-His tag, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Not tested: Met predicted.
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl, Sucrose and DTT.
• Bio-activity: The activity of the activated Caspase-3 was measured by its ability to cleave DEVD-AFC. The specific activity is >500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CASP3, Met&His-tagged has a calculated MW of 33 kDa. In SDS-PAGE migrates as 35 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: Met1-His277

Gene Information

• Gene Name: CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis related cysteine protease; caspase-3; apopain; CPP32; CPP32B; Yama; CASP-3; CPP-32; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; SCA-1
• Gene ID: 836
• mRNA Refseq: NM_004346
• Protein Refseq: NP_004337
• MIM: 600636
• UniProt ID: P42574
• Chromosome Location: 4q34

Reviews

03/29/2024

Casp3-748R, the rat caspase3 worked/is working very well for us.