Active Recombinant Human CCL2 Protein (Gln24-Thr99), N-His tagged, Animal-free, Carrier-free

• Cat.No.: CCL2-267H
• Product Overview: Recombinant Human CCL2 Protein (Gln24-Thr99) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: C-C Motif Chemokine Ligand 2 (CCL2) is an 8.44 kDa cytokine with 76 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1). The CCL2 secreted from monocytes, macrophages, and dendritic cells. Upon binding with CCR4, it regulates many biological functions, such as recruitments of monocytes, memory T cells, and dendritic cells, response to TNF-α and Interferon-γ, angiogenesis, cellular homeostasis, and cytoskeleton organization.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A. The ED50 for this effect is < 20 ng/mL.
• Molecular Mass: The protein has a calculated MW of 9.49 kDa.
  The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gln24-Thr99
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: CCL2 chemokine (C-C motif) ligand 2 [ Homo sapiens (human) ]
• Official Symbol: CCL2
• Synonyms: CCL2; chemokine (C-C motif) ligand 2; SCYA2, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); C-C motif chemokine 2; GDCF 2; HC11; MCAF; MCP 1; MCP1; MGC9434; monocyte chemoattractant protein 1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; homologous to mouse Sig je; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys Cys); member 2; SMC CF; small-inducible cytokine A2; monocyte chemoattractant protein-1; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Gene ID: 6347
• mRNA Refseq: NM_002982
• Protein Refseq: NP_002973
• MIM: 158105
• UniProt ID: P13500