Active Recombinant Human CD160 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD160-453HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD160 (NP_008984.1) (Met 1-Leu 158) without the propeptide was expressed, with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 143
• Form: Lyophilized
• Bio-activity: Measured by its ability to bind with biotinylated human HVEM-Fch in a functional ELISA.
• Molecular Mass: The recombinant human CD160 consists of 143 amino acids and has a predicted molecular mass of 16.4 kDa. In SDS-PAGE under reducing conditions, rh CD160 migrates as an approximately 25 kDa band due to glycosylation.
• N-terminal Sequence Analysis: Ile 27
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: CD160 CD160 molecule [ Homo sapiens ]
• Official Symbol: CD160
• Synonyms: CD160; CD160 molecule; CD160 antigen; BY55; NK1; NK28; CD160-delta Ig; CD160 transmembrane isoform; natural killer cell receptor BY55; natural killer cell receptor, immunoglobulin superfamily member; FLJ46513
• Gene ID: 11126
• mRNA Refseq: NM_007053
• Protein Refseq: NP_008984
• MIM: 604463
• UniProt ID: O95971