Active Recombinant Human CD16a protein, His-tagged, Biotinylated
| Cat.No. : | CD16a-0235H |
| Product Overview : | Biotinylated Recombinant Human CD16a protein(P08637-1)(Met1-Gln208,F176V), fused with His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
| Source : | HEK293 |
| Species : | Human |
| Tag : | His |
| Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : | 1.Immobilized Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) at 10 μg/mL can bind recombinant human IgG1 (Fc) with a linear range of 0.31-5.0 μg/mL.2.Loaded Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 3.3 μM as determined in a BLI assay (ForteBio Octet Red384).3.Loaded Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Conbercept with an affinity constant of 0.1 μM as determined in a BLI assay. |
| Molecular Mass : | The secreted recombinant human CD16a consists of 227 amino acids and predicts a molecular mass of 25.6 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 KDa due to glycosylation. |
| Protein Length : | Met1-Gln208,F176V |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Official Symbol : | CD16a |
| Official Symbol : | CD16a |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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