Active Recombinant Human CD22 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD22-561HAF555
• Product Overview: The Alexa Fluor 555 conjugated recombinant human CD22-Fc fusion protein is expressed as an 892 amino acid protein consisting of Asp20-Thr683 region of CD22 (UniProt accession #P20273) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Bio-activity: Immobilized CD22 ECD protein supports the adhesion of human red blood cells. Binds anti-CD22 monoclonal antibodies with high affinity.
• Molecular Mass: Calculated molecular mass: 100.2 kDa
  Estimated by SDS-PAGE under reducing condition: 120-130 kDa
• AA Sequence: DSSKWVFEHPETLYAWEGACVWIPC TYRALDGDLESFILFHNPEYNKNTS KFDGTRLYESTKDGKVPSEQKRVQF LGDKNKNCTLSIHPVHLNDSGQLGL RMESKTEKWMERIHLNVSERPFPPH IQLPPEIQESQEVTLTCLLNFSCY GYPIQLQWLLEGVPMRQAAVTSTSL TIKSVFTRSELKFSPQWSHHGKIVT CQLQDADGKFLSNDTVQLNVKHTP KLEIKVTPSDAIVREGDSVTMTCEV SSSNPEYTTVSWLKDGTSLKKQNTF TLNLREVTKDQSGKYCCQVSNDVG PGRSEEVFLQVQYAPEPSTVQILHS PAVEGSQVEFLCMSLANPLPTNYTW YHNGKEMQGRTEEKVHIPKILPWH AGTYSCVAENILGTGQRGPGAELDV QYPPKKVTTVIQNPMPIREGDTVTL SCNYNSSNPSVTRYEWKPHGAWEE PSLGVLKIQNVGWDNTTIACAACNS WCSWASPVALNVQYAPRDVRVRKIK PLSEIHSGNSVSLQCDFSSSHPKE VQFFWEKNGRLLGKESQLNFDSISP EDAGSYSCWVNNSIGQTASKAWTLE VLYAPRRLRVSMSPGDQVMEGKSA TLTCESDANPPVSHYTWFDWNNQSL PYHSQKLRLEPVKVQHSGAYWCQGT NSVGKGRSPLSTLTVYYSPETSTG THTCPPCPAPELLGGPSVFLFPPKP KDTLMISRTPEVTCVVVDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQY NSTYRVVSVLTVLHQDWLNGKEYKC KVSNKALPAPIEKTISKAKGQPREP QVYTLPPSREEMTKNQVSLTCLVK GFYPSDIAVEWESNGQPENNYKTTP PVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLS PGK
• Endotoxin: < 0.1 EU/ μg of purified recombinant protein determined by the LAL method
• Purity: > 90 % by SDS-PAGE under reducing condition
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: The product is shipped at 4 centigrade. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied at 0.5 mg/ml in sterile PBS pH 7.4 (carrier & preservative free).

Gene Information

• Gene Name: CD22 CD22 molecule [ Homo sapiens ]
• Official Symbol: CD22
• Synonyms: CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; sialic acid binding Ig like lectin 2; SIGLEC 2; SIGLEC2; BL-CAM; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2; SIGLEC-2; FLJ22814; MGC130020
• Gene ID: 933
• mRNA Refseq: NM_001185099
• Protein Refseq: NP_001172028
• MIM: 107266
• UniProt ID: P20273