Species : |
Human |
Source : |
HEK293 |
Tag : |
mFc |
Protein Length : |
Leu 24-Gln 186 |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human 4‑1BB Ligand, Fc Tag at 1 μg/mL (100 μL/well) can bind Human 4-1BB Protein, Mouse IgG2a Fc Tag with a linear range of 0.6-5 ng/mL.Immobilized Human 4‑1BB Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Human 4-1BB Protein, Mouse IgG2a Fc Tag with a linear range of 0.1-4 ng/mL.Immobilized Human 4-1BB Protein, Mouse IgG2a Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.2-6 ng/mL. |
Molecular Mass : |
This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 44.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |