Active Recombinant Human CD27 Protein, Fc-tagged, Alexa Fluor 488 conjugated
Cat.No. : | CD27-180HAF488 |
Product Overview : | Alexa Fluor 488 conjugated recombinant human CD27 (P26842) (Met1-Ile192) was expressed, fused with the Fc region of rabbit IgG at the C-terminus. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized human CD27-his at 10 μg/mL (100 μL/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL. |
Molecular Mass : | The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions. |
Protein length : | 396 |
N-terminal Sequence Analysis : | Ala 20 |
Endotoxin : | < 1.0 EU/ μg of the protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 488 via amines Excitation Wavelength: 488 nm Emission Wavelength: 515-545 nm |
Stability : | Samples are stable for up to 12 months from date of receipt at -70 centigrade. |
Storage : | Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Storage Buffer : | Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | CD27 CD27 molecule [ Homo sapiens ] |
Official Symbol : | CD27 |
Synonyms : | CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393; |
Gene ID : | 939 |
mRNA Refseq : | NM_001242 |
Protein Refseq : | NP_001233 |
MIM : | 186711 |
UniProt ID : | P26842 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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