Active Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin

• Cat.No.: CD274-379H
• Product Overview: Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1). Predicted N-terminus: Phe 19. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 65-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: lIgG2b
• Protein Length: Phe 19- Arg 238
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-1, His Tag at 1 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 0.078-2.5 μg/mL (QC tested).
  Immobilized Recombinant Human CD274 Protein, LIgG2b Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc Tag with a linear range of 20-313 ng/mL (Routinely tested).
• Molecular Mass: 53.0 kDa
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7