Active Recombinant Human CD28, HIgG1 Fc-tagged

• Cat.No.: CD28-378H
• Product Overview: The extracellular domain of human CD28 (AAA60581.1) (Asn19-Pro152) is fused to the N-terminus of the Fc region of human IgG1 was expressed in CHO cell.

Specification

• Description: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, andT-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms havebeen found for this gene.
• Source: CHO cells
• Species: Human
• Tag: HIgG1 Fc-tagged
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells.
• Molecular Mass: 41 KDa (monomer)
• AA Sequence: Asn19-Pro152
• Endotoxin: <0.06 eu/μg as determined by lal test.>
• Purity: >98%, by SDS-PAGE under reducing conditions.
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747
• Chromosome Location: 2q33
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem
• Function: SH3/SH2 adaptor activity; coreceptor activity; identical protein binding; protease binding; protein binding; protein homodimerization activity