Active Recombinant Human CD33 Protein, Fc-tagged, Alexa Fluor® 647 conjugated
Cat.No. : | CD33-26H |
Product Overview : | Recombinant human Alexa Fluor® 647 conjugated Siglec-3 (Asp18-His259)(Val257Leu) protein with human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in mouse myeloma cell line. |
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Description : | Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome. |
Source : | Mouse myeloma cell line |
Species : | Human |
Tag : | Fc |
Form : | Disulfide-linked homodimer Labeled with Alexa Fluor® 647 via amines Excitation Wavelength: 650 nm Emission Wavelength: 668 nm |
Bio-activity : | Measured by flow cytometry for its ability to bind anti-human Siglec-3/CD33 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Siglec-3/CD33 Fc Chimera Alexa Fluor® 647 that produces 50% of the binding response is 0.50-20.0 ng/mL. |
Molecular Mass : | 53.4 kDa |
N-terminal Sequence Analysis : | Asp18 |
Endotoxin : | <1.0 EU/μg of the protein by the LAL method. |
Purity : | >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after opening. 3 months, -20 to -70 centigrade under sterile conditions after opening. |
Storage Buffer : | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Shipping : | The product is shipped with dry ice or equivalent. |
Gene Name : | CD33 CD33 molecule [ Homo sapiens (human) ] |
Official Symbol : | CD33 |
Synonyms : | CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; CD33 antigen (gp67); CD33 molecule transcript; gp67; sialic acid-binding Ig-like lectin 3 |
Gene ID : | 945 |
mRNA Refseq : | M23197 |
Protein Refseq : | AAA51948 |
MIM : | 159590 |
UniProt ID : | P20138 |
Products Types
◆ Recombinant Protein | ||
CD33-0791H | Recombinant Human CD33 Protein, GST-Tagged | +Inquiry |
CD33-670H | Recombinant Human CD33 Protein, His-tagged | +Inquiry |
CD33-190H | Recombinant Human CD33 Protein, His-tagged | +Inquiry |
CD33-28H | Active Recombinant Human CD33 Protein, Fc-tagged, Atto 647N conjugated | +Inquiry |
CD33-3097H | Recombinant Human CD33 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
CD33-1808MCL | Recombinant Mouse CD33 cell lysate | +Inquiry |
CD33-978HCL | Recombinant Human CD33 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewQuick dissolution, easy use.
Excellent solubility, no aggregates.
High concentration, very effective.
Q&As (7)
Ask a questionCertain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.
CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.
CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.
CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.
While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.
CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.
The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.
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