Recombinant Mouse Cd33 Antigen Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: Cd33-6889MAF647
• Product Overview: A DNA sequence encoding the extracellular domain of mouse Cd33 (Met 1 - Glu 240) (Alexa Fluor 647 conjugated) was fused with the Fc region of human IgG1 at the C-terminus. It comprises 465 amino acids and has a calculated molecular mass of 51.6 kDa.

Specification

• Description: Mouse myeloid cell surface antigen Cd33 is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Cd33 contains 1 Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain.
• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The secreted recombinant mouse Cd33/Fc is a disulfide-linked homodimer. The reduced monomer comprises 465 amino acids and has a calculated molecular mass of 51.6 kDa. As a result of glycosylation, the apparent molecular mass of rm CD33/Fc monomer is approximately 55 - 60 kDa in SDS-PAGE under reducing conditions.
• Protein length: 465
• N-terminal Sequence Analysis: Gln 17
• Endotoxin: < 0.06 EU/ μg as determined by LAL test.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: Cd33 CD33 antigen [ Mus musculus ]
• Official Symbol: Cd33
• Synonyms: CD33; CD33 antigen; myeloid cell surface antigen CD33; sialic acid-binding Ig-like lectin 3; gp67; Siglec-3
• Gene ID: 12489
• mRNA Refseq: NM_001111058
• Protein Refseq: NP_001104528

Reviews

11/14/2019

Quick dissolution, easy use.

08/27/2019

Excellent solubility, no aggregates.

02/27/2019

High concentration, very effective.

Q&As

What are the implications of CD33 polymorphisms in therapeutic targeting?

Certain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.

What is the primary biological function of CD33 in hematopoietic cells?

CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.

How does CD33 expression vary among different leukocyte populations?

CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.

How has CD33 been utilized as a therapeutic target in hematological malignancies?

CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.

How does CD33 signaling influence myeloid cell differentiation?

While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.

What role does CD33 play in acute myeloid leukemia (AML)?

CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.

Are there known ligands that bind specifically to CD33?

The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.