Active Recombinant Human CD3E Protein, Fc Tagged, Low endotoxin

• Cat.No.: CD3E-11182H
• Product Overview: Human CD3 epsilon (23-48) Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23-Thr 48 (Accession # P07766). This protein carries a human IgG1 Fc tag at the C-terminus. Predicted N-terminus: Asp 23

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 23-48 aa
• Description: The Asp23-Thr48 region of human CD3E is a core part of its extracellular immunoglobulin-like (Ig-like) domain. Its primary function is to act as a crucial structural scaffold, mediating proper interactions with other CD3 subunits (specifically CD3G and CD3D). It is considered as SP34 binding site.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human CD3 epsilon (23-48) Protein, Fc Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) with a linear range of 0.06-4 ng/mL.
• Molecular Mass: The protein has a calculated MW of 29.2 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.01 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens (human) ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• MIM: 186830
• UniProt ID: P07766