Recombinant Mouse CD3E & CD3G protein(Met1-Asp108 & Met1-Ser116), hFc&His&hFc-tagged

• Cat.No.: CD3E & CD3G-3257M
• Product Overview: Recombinant extracellular domain (Met1-Asp108) of Mouse CD3E (NP_031674.1) was expressed in HEK293, fused with the C-terminal polyhistidine tagged Fc region of Human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Ser116) of Mouse CD3G (NP_033980.1) was fused with the Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse CD3E/CD3G heterodimer was purified.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc&His&hFc
• Protein length: Met1-Asp108 & Met1-Ser116
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of mouse CD3E/CD3G comprises 671 (336+335) amino acids and has a calculated molecular mass of 75.4 (38.1+37.3) KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: 95.1 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.