Active Recombinant Human CD44 protein, His-tagged

• Cat.No.: CD44-3961H
• Product Overview: Recombinant Human CD44 protein(Gln 21-Pro 220), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln 21-Pro 220
• Tag: C-His
• Form: Liquid in sterile PBS, pH7.4.
• Bio-activity: Immobilized Human CD44, His Tag at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 39-156 ng/mL.
• Molecular Mass: The protein has a calculated MW of 22.9 kDa. The protein migrates as 35-58 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 1.0mg/ml.
• Reconstitution: Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Disruption of the pentraxin 3/CD44 interaction as an efficient therapy for triple-negative breast cancers (2021)
  Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications (2018)

Gene Information

• Gene Name: CD44 CD44 molecule (Indian blood group) [ Homo sapiens ]
• Official Symbol: CD44
• Synonyms: CD44; CD44 molecule (Indian blood group); CD44 antigen (homing function and Indian blood group system) , MDU2, MDU3, MIC4; CD44 antigen; CD44R; chondroitin sulfate proteoglycan 8; CSPG8; HCELL; hematopoietic cell E and L selectin ligand; IN; MC56; Pgp1; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system; LHR; MDU2; MDU3; MIC4; CDW44; HUTCH-I; ECMR-III; MGC10468
• Gene ID: 960
• mRNA Refseq: NM_000610
• Protein Refseq: NP_000601
• MIM: 107269
• UniProt ID: P16070

Citation

Injectable Hyaluronic Acid- co -Gelatin Cryogels for Tissue-Engineering Applications

Journal: Materials    PubMed ID: 30087295    Data: 2018/8/7

Authors: Mahboobeh Rezaeeyazdi, Thibault Colombani, Sidi A. Bencherif

Article Snippet:Briefly, cryogels were saturated for 1 h at RT with 1X PBS + 0.05% Tween-20 + 1% bovine serum albumin (BSA).Briefly, cryogels were saturated for 1 h at RT with 1X PBS + 0.05% Tween-20 + 1% bovine serum albumin (BSA).. Next, cryogels were incubated for 3 h at RT with either 10μg/mL of recombinant human CD44 His-tagged (CD44-3961H, Creative BioMart, Shirley, NY, USA) or anti-porcine gelatin anti-serum (GELP12-S, Alpha Diagnostic International, San Antonio, TX, USA) diluted 1:100 in 1X PBS + 0.05% Tween-20 + 1% BSA.. Next, cryogels were treated for 1 h at RT in the dark with Alexa Fluor 488-conjugated 6x-His Tag monoclonal antibody (Fisher Scientific) or FITC-conjugated anti-rabbit IgG (Fc-region) (Alpha Diagnostic International), respectively.Next, cryogels were treated for 1 h at RT in the dark with Alexa Fluor 488-conjugated 6x-His Tag monoclonal antibody (Fisher Scientific) or FITC-conjugated anti-rabbit IgG (Fc-region) (Alpha Diagnostic International), respectively.

Evaluation of biological properties of cryogels. Immunostaining of HAGM, MA-gelatin, HA- co -Gelatin, and MA-alginate cryogels using human CD44 His-tagged receptor and anti-gelatin antibody. CD44 binding was depicted with an Alexa Fluor 488-conjugated 6x-His Tag monoclonal antibody (green), and anti-gelatin antibody binding was detected with a FITC-conjugated anti-rabbit IgG (green) (scale bar = 100 μm).