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Active Recombinant Human CD47 Protein, His-tagged, Biotinylated

Cat.No. : CD47-7891H
Product Overview : Biotinylated Recombinant Human CD47 Protein (Gln 19 - Pro 139) (NP_942088), fused with a polyhistidine tag at the C-terminus, followed by an Avi tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln 19
Form : Lyophilized from sterile PBS, pH7.4, 10% trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Anti-CD47 mAb, Human IgG4 at 5μg/mL (100 μL/well) can bind Biotinylated Human CD47, His Tag with a linear range of 0.039-1.25 μg/mL.
Molecular Mass : Recombinant Human CD47 Protein has a calculated MW of 16.4 kDa. The protein migrates as 34-48 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length : Gln 19 - Pro 139
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : CD47 CD47 molecule [ Homo sapiens ]
Official Symbol : CD47
Synonyms : CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6;
Gene ID : 961
mRNA Refseq : NM_001777
Protein Refseq : NP_001768
MIM : 601028
UniProt ID : Q08722
Chromosome Location : 3q13.1-q13.2
Pathway : Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Integrins in angiogenesis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem;
Function : protein binding; thrombospondin receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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