Active Recombinant Human CD47 Protein, His-tagged, Biotinylated

• Cat.No.: CD47-7891H
• Product Overview: Biotinylated Recombinant Human CD47 Protein (Gln 19 - Pro 139) (NP_942088), fused with a polyhistidine tag at the C-terminus, followed by an Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Gln 19
• Form: Lyophilized from sterile PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-CD47 mAb, Human IgG4 at 5μg/mL (100 μL/well) can bind Biotinylated Human CD47, His Tag with a linear range of 0.039-1.25 μg/mL.
• Molecular Mass: Recombinant Human CD47 Protein has a calculated MW of 16.4 kDa. The protein migrates as 34-48 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Protein length: Gln 19 - Pro 139
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD47 CD47 molecule [ Homo sapiens ]
• Official Symbol: CD47
• Synonyms: CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6
• Gene ID: 961
• mRNA Refseq: NM_001777
• Protein Refseq: NP_001768
• MIM: 601028
• UniProt ID: Q08722
• Chromosome Location: 3q13.1-q13.2
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Integrins in angiogenesis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem
• Function: protein binding; thrombospondin receptor activity