Species : |
Human |
Source : |
HEK293 |
Tag : |
Flag&His&mFc |
Protein Length : |
Leu 33-Arg 143 (CD79A) & Ala 29-Asp 159 (CD79B) |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant. |
Bio-activity : |
Immobilized Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag at 1 μg/mL (100 μL/well) can bind Polatuzumab with a linear range of 0.1-1 ng/mL. |
Molecular Mass : |
Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag is produced by co-expression of CD79A and CD79B, has a calculated MW of 43.8 kDa (CD79A) and 46.7 kDa (CD79B). Subunit CD79A carries a mouse IgG2a Fc fragment at the C-terminus, followed by a flag tag and subunit CD79B carries a mouse IgG2a Fc fragment at the C-terminus, followed by a polyhistidine tag. The non-reducing (NR) protein migrates as 115-120 kDa due to glycosylation. |
Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |