Active Recombinant Human CD79A & CD79B protein, mFc-Flag &mFc-His-tagged

• Cat.No.: CD79A & CD79B-2956H
• Product Overview: Recombinant Human CD79A&CD79B protein(Leu 33-Arg 143 (CD79A) & Ala 29-Asp 159 (CD79B)), fused with mFc-Flag and mFc-His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc-Flag&mFc-His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag at 1 μg/mL (100 μL/well) can bind Polatuzumab with a linear range of 0.1-1 ng/mL.
• Molecular Mass: Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag is produced by co-expression of CD79A and CD79B, has a calculated MW of 43.8 kDa (CD79A) and 46.7 kDa (CD79B). Subunit CD79A carries a mouse IgG2a Fc fragment at the C-terminus, followed by a flag tag and subunit CD79B carries a mouse IgG2a Fc fragment at the C-terminus, followed by a polyhistidine tag. The non-reducing (NR) protein migrates as 115-120 kDa due to glycosylation.
• Protein Length: Leu 33-Arg 143 (CD79A) & Ala 29-Asp 159 (CD79B)
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD79A CD79a molecule, immunoglobulin-associated alpha [ Homo sapiens ]
• Official Symbol: CD79A
• Synonyms: CD79A; CD79a molecule, immunoglobulin-associated alpha; CD79A antigen (immunoglobulin associated alpha) , IGA; B-cell antigen receptor complex-associated protein alpha chain; MB 1; ig-alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; CD79a antigen (immunoglobulin-associated alpha); membrane-bound immunoglobulin-associated protein; IGA; MB-1
• Gene ID: 973
• mRNA Refseq: NM_001783
• Protein Refseq: NP_001774
• MIM: 112205
• UniProt ID: P11912

Reviews

05/26/2019

The operational approach for this reagent is clear and comprehensible, avoiding any complexity in experimental steps or techniques.

07/18/2018

With stringent quality control measures, each batch of this protein reagent is subjected to rigorous evaluation to ensure consistent quality.

03/07/2016

Its excellent reproducibility allows for repeated experimental verification.