Active Recombinant Human CD8A Protein, Fc Tagged, Low endotoxin

• Cat.No.: CD8A-11215H
• Product Overview: Human CD8 alpha Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ser 22-Asp 182 (Accession # P01732-1). This protein carries a human IgG1 Fc tag at the C-terminus. Predicted N-terminus: Ser 22

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 22-182 aa
• Description: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human CD8 alpha Protein, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.2-8 ng/mL.
• Molecular Mass: The protein has a calculated MW of 44.1 kDa. The protein migrates as 53-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.01 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD8A CD8a molecule [ Homo sapiens (human) ]
• Official Symbol: CD8A
• Synonyms: CD8A; CD8a molecule; CD8, CD8 antigen, alpha polypeptide (p32), T cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; p32; Leu2
• Gene ID: 925
• mRNA Refseq: NM_001145873
• Protein Refseq: NP_001139345
• MIM: 186910
• UniProt ID: P01732