Active Recombinant Human CD8A protein, His-tagged

• Cat.No.: CD8A-3196H
• Product Overview: Active Recombinant Human CD8A protein(P01732-1)(Ser22-Asp182) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-182 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human CD8 alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.70 kDa. Due to glycosylation, the protein migrates to 28-32 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001139345.1
• Unit ID: P01732
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P01732
• Publications:
  • Aptamer-Containing Surfaces for Selective Capture of CD4 Expressing Cells (2012)

Gene Information

• Gene Name: CD8A CD8a molecule [ Homo sapiens ]
• Official Symbol: CD8A
• Synonyms: CD8A; CD8a molecule; CD8, CD8 antigen, alpha polypeptide (p32) , T cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; p32; Leu2
• Gene ID: 925
• mRNA Refseq: NM_001145873
• protein Refseq: NP_001139345
• MIM: 186910