Active Recombinant Human CD97 protein, Fc-tagged

• Cat.No.: CD97-3134H
• Product Overview: Active Recombinant Human CD97 protein(Met 1-Gln 398)(NP_001775.2) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-398 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD55 at 2 μg/ml (100 μl/well) can bind human CD97 with a linear ranger of 1.28-32 ng/ml.
• Molecular Mass: The recombinant human CD97/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 619 amino acids and predictes a molecular mass of 68.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD97/Fc monomer is approximately 100-110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD97 CD97 molecule [ Homo sapiens ]
• Official Symbol: CD97
• Synonyms: CD97; CD97 molecule; CD97 antigen; leukocyte antigen CD97; seven transmembrane helix receptor; seven span transmembrane protein; seven transmembrane; heterodimeric receptor associated with inflammation; TM7LN1; seven-span transmembrane protein; seven-transmembrane, heterodimeric receptor associated with inflammation
• Gene ID: 976
• mRNA Refseq: NM_001025160
• Protein Refseq: NP_001020331
• MIM: 601211
• UniProt ID: P48960