Active Recombinant Human CDH1, Fc tagged

• Cat.No.: CDH1-274H
• Product Overview: Recombinant Human CDH1 (P12830) (Met1-Ile707), fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ile707
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), > 30% will adhere specifically after 90 minutes at 37 °C.
• Molecular Mass: The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion (2017)
  Cholesterol metabolism and statin effects on an FH class II LDL-receptor mutation (2018)

Gene Information

• Gene Name: CDH1 cadherin 1, type 1, E-cadherin (epithelial) [ Homo sapiens ]
• Official Symbol: CDH1
• Synonyms: CDH1; cadherin 1, type 1, E-cadherin (epithelial); UVO; cadherin-1; CD324; E Cadherin; uvomorulin; CAM 120/80; E-Cadherin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; CDHE; ECAD; LCAM; Arc-1
• Gene ID: 999
• mRNA Refseq: NM_004360
• Protein Refseq: NP_004351
• MIM: 192090
• UniProt ID: P12830

Citation

A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion

Journal: Nature cell biology    PubMed ID: 28218910    Data: 2018/3/1

Authors: Anna Labernadie, Takuya Kato, Xavier Trepat

Article Snippet:Bead pulling experiments were performed using magnetic tweezers as previously described 19 , 66 .Bead pulling experiments were performed using magnetic tweezers as previously described 19 , 66 .. Briefly, 4.47 μm diameter ferromagnetic beads with carboxyl surface groups (Spherotech, Lake Forest, IL) were covalently coated with a 32μg/ml solution of purified E-cadherin–Fc (Creative BioMart, #CDH1-274H), P-cadherin–Fc (R&D Systems, #861-PC) or N-cadherin–Fc (Creative BioMart, #CDH2-315H) proteins.. Beads were first washed with Na phosphate buffer (0.1 M, pH 8), incubated with 32 μg/ml of the Fc-tagged proteins for 5 h at 4oC and then with crosslinking buffer for 1 h (25 mM DMP, 0.2 M triethanolamine, pH 8.2).Beads were first washed with Na phosphate buffer (0.1 M, pH 8), incubated with 32 μg/ml of the Fc-tagged proteins for 5 h at 4oC and then with crosslinking buffer for 1 h (25 mM DMP, 0.2 M triethanolamine, pH 8.2).