Active Recombinant Human CDK1 & CCNE1 Protein, GST & His-tagged

• Cat.No.: CDK1 & CCNE1-1248H
• Product Overview: Recombinant human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; Recombinant human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the pla

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-297;1-410 a.a.
• Predicted N Terminal: Met & His
• Form: Supplied as sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.
• Bio-activity: The specific activity was determined to be 150 nmol/min/mg using Histone H1 as substrate.
• Molecular Mass: The recombinant heterotrimer of human CDC2 & CCNE1 comprises 950 (522+428) amino acids and has a calculated molecular mass of 109.7 (60.4+49.3) kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CDK1 cyclin-dependent kinase 1 [ Homo sapiens ]
• Official Symbol: CDK1
• Synonyms: CDC2; CDC28A; P34CDC2; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase
• Gene ID: 983
• mRNA Refseq: NM_001170406
• Protein Refseq: NP_001163877
• MIM: 116940
• Chromosome Location: 10q21.1
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Centrosome maturation, organism-specific biosystem
• Function: ATP binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding