Active Recombinant Human CDK2/CCNE1 protein, GST-tagged

• Cat.No.: CDK2-48H
• Product Overview: Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 404 nmol/min/mg
• Molecular Mass: CDK2 ~58kDa and CyclinE1 ~73kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Stability: 1 year at -70 centigrade from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: CDK2 cyclin-dependent kinase 2 [ Homo sapiens ]
• Official Symbol: CDK2
• Synonyms: CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2)
• Gene ID: 1017
• mRNA Refseq: NM_001798
• Protein Refseq: NP_001789
• MIM: 116953
• UniProt ID: P24941
• Chromosome Location: 12q13
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of ATR in response to replication stress, organism-specific biosystem
• Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase activity; contributes_to histone kinase activity; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity