Active Recombinant Human CEACAM5 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CEACAM5-87HB
• Product Overview: Biotinylated Recombinant Human CEACAM5 protein(Q8N4D0)(Lys35-Ala685), fused to C-terminal His tag and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on Tris-Bis PAGE result.
• Protein length: Lys35-Ala685
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  '-20 to -80°C for 3-6 months in unopened state after reconstitution.
  4-8℃ for 2-7 days after reconstitution.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5 [ Homo sapiens ]
• Official Symbol: CEACAM5
• Synonyms: CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100; DKFZp781M2392
• Gene ID: 1048
• mRNA Refseq: NM_004363
• Protein Refseq: NP_004354
• MIM: 114890
• UniProt ID: P06731