Active Recombinant Human CERK, Flag-tagged
Cat.No. : | CERK-510H |
Product Overview : | Recombinant Human CERK, fused with C-terminal Flag tag, was expressed in Sf9 cells. |
- Specification
- Gene Information
- Related Products
Description : | CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation. |
Source : | Sf9 cells |
Species : | Human |
Tag : | Flag |
Form : | Provided in 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 µg/ml Flag peptide, 3 mM DTT and 20% glycerol |
Bio-activity : | 142 pmole/min/μg. Assay conditions: CERK activity was measured at 37 oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI). |
Molecular Mass : | 87 kDa |
Purity : | 0.8 |
Applications : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling |
Storage : | Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles. |
Gene Name : | CERK ceramide kinase [ Homo sapiens ] |
Official Symbol : | CERK |
Synonyms : | CERK; LK4; hCERK; dA59H18.2; dA59H18.3; ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase; NP_073603.2; EC 2.7.1.138 |
Gene ID : | 64781 |
mRNA Refseq : | NM_022766 |
Protein Refseq : | NP_073603 |
MIM : | 610307 |
UniProt ID : | Q8TCT0 |
Chromosome Location : | 22q13.31 |
Pathway : | Glycosphingolipid metabolism; Integrated Breast Cancer Pathway; Sphingolipid Metabolism |
Function : | ATP binding; NAD+ kinase activity; ceramide kinase activity |
Products Types
◆ Recombinant Protein | ||
CERK-1598M | Recombinant Mouse CERK Protein, His (Fc)-Avi-tagged | +Inquiry |
CERK-745H | Recombinant Human CERK Protein, His-tagged | +Inquiry |
CERK-1147H | Recombinant Human CERK Protein, GST-Tagged | +Inquiry |
CERK-648R | Recombinant Rhesus Macaque CERK Protein, His (Fc)-Avi-tagged | +Inquiry |
CERK-3319M | Recombinant Mouse CERK Protein | +Inquiry |
◆ Lysates | ||
CERK-7567HCL | Recombinant Human CERK 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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