| Description : |
Carboxylesterase 1, also known as liver carboxylesterase 1 isoform a, is a member of a large family of carboxylesterases. It is also part of the alpha/beta fold hydrolase family. Carboxylesterases hydrolyze long-chain fatty acid esters and thioesters. The resulting carboxylates are then often conjugated by other enzymes to increase solubility and eventually excreted. Also, this enzyme may play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds. This protein is known to react aromatic and aliphatic esters and can manage cellular cholesterol esterification levels. It is present in most tissues with higher levels in the liver and low levels in the gastrointestinal tract. Recombinant human Carboxylesterase 1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Source : |
Insect cell |
| Species : |
Human |
| Tag : |
His |
| Form : |
Liquid. In 25 mM Sodium Acetate (pH 4.0) containing 10 % glycerol, 0.1 M NaCl, 0.1 mM PMSF. |
| Bio-activity : |
Specific activity is > 700 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37 centigrade. |
| Molecular Mass : |
61.7 kDa |
| Protein length : |
19-568 aa |
| AA Sequence : |
ADLGHPSSPP VVDTVHGKVL GKFVSLEGFA QPVAIFLGIP FAKPPLGPLR FTPPQPAEPW SFVKNATSYP PMCTQDPKAG QLLSELFTNR KENIPLKLSE DCLYLNIYTP ADLTKKNRLP VMVWIHGGGL MVGAASTYDG LALAAHENVV VVTIQYRLGI WGFFSTGDEH SRGNWGHLDQ VAALRWVQDN IASFGGNPGS VTIFGESAGG ESVSVLVLSP LAKNLFHRAI SESGVALTSV LVKKGDVKPL AEQIAITAGC KTTTSAVMVH CLRQKTEEEL LETTLKMKFL SLDLQGDPRE SQPLLGTVID GMLLLKTPEE LQAERNFHTV PYMVGINKQE FGWLIPMQLM SYPLSEGQLD QKTAMSLLWK SYPLVCIAKE LIPEATEKYL GGTDDTVKKK DLFLDLIADV MFGVPSVIVA RNHRDAGAPT YMYEFQYRPS FSSDMKPKTV IGDHGDELFS VFGAPFLKEG ASEEEIRLSK MVMKFWANFA RNGNPNGEGL PHWPEYNQKE GYLQIGANTQ AAQKLKDKEV AFWTNLFAKK AVEKPPQTEH IELHHHHHH |
| Endotoxin : |
< 1.0 EU per 1 ug of protein (determined by LAL method) |
| Purity : |
> 90 % by SDS - PAGE |
| Notes : |
For research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
| Storage : |
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.5 mg/ml (determined by Absorbance at 280 nm) |
