Active Recombinant Human CHEK2, GST-tagged

• Cat.No.: CHEK2-141H
• Product Overview: Recombinant human CHEK2 (amino acid residues 5-543), with N-terminal GST and C-terminal 6His, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 5-543 a.a.
• Description: The checkpoint kinases CHK1 and CHK2 are serine/threonine protein kinases that are key components of the intracellular signalling network which responds to damaged DNA, and co-ordinates activation of cell cycle checkpoints with initiation and implementation of DNA repair.
• Form: 50 mM Tris/HCl pH7.5, 0.1mM EGTA, 150 mM NaCl, 0.1% ?-Mercaptoethanol, 270 mM sucrose, 0.03% Brij-35, 1 mM Benzamidine, 0.2 mM PMSF.
• Bio-activity: 621.6 Units/mg (571.8 Units/ml)Substrate: CHKtide (KKKVSRSGLYRSPSMPENLNRPR)
• Molecular Mass: ~89.5 kDa
• Purity: >85% by InstantBlue™ SDS-PAGE
• Unit Definition: 1 Unit = 1 nmole of phosphate incorporated into the substrate in 1 minute.
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.92 mg/ml

Gene Information

• Gene Name: CHEK2 checkpoint kinase 2 [ Homo sapiens ]
• Official Symbol: CHEK2
• Synonyms: CHEK2; checkpoint kinase 2; CHK2 (checkpoint, S.pombe) homolog , CHK2 checkpoint homolog (S. pombe) , RAD53; serine/threonine-protein kinase Chk2; bA444G7; CDS1; CHK2; HuCds1; PP1425; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2; LFS2; RAD53; hCds1
• Gene ID: 11200
• mRNA Refseq: NM_001005735
• Protein Refseq: NP_001005735
• UniProt ID: O96017
• Chromosome Location: 22q12.1
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; FOXM1 transcription factor network, organism-specific biosystem; G1/S DNA Damage Checkpoints, organism-specific biosystem
• Function: ATP binding; metal ion binding; nucleotide binding; protein binding; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity