Active Recombinant Human CLDN18 protein

• Cat.No.: CLDN18-059H
• Product Overview: Recombinant Human CLDN18 protein(Met 1 - Val 261) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Form: The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Fluorescent Human Claudin-18.2 Full Length Protein-VLP (HEK293) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL.
• Molecular Mass: This protein carries a GFP tagVirus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening. The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
• Protein Length: Met 1 - Val 261
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.