Active Recombinant Human CLK2 Protein, GST-tagged

• Cat.No.: CLK2-1493H
• Product Overview: Human CLK2 (AAH53603.1, 1 a.a. - 499 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
• Source: Insect Cells
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: DYRKtide-F. ATP: 100 uM.
• Molecular Mass: 87 kDa
• Purity: 69 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CLK2 CDC-like kinase 2 [ Homo sapiens ]
• Official Symbol: CLK2
• Synonyms: CLK2; CDC-like kinase 2; dual specificity protein kinase CLK2; clk2; CLK kinase; hCLK2; MGC61500
• Gene ID: 1196
• mRNA Refseq: NM_003993
• Protein Refseq: NP_003984
• MIM: 602989
• UniProt ID: P49760