Active Recombinant Human COL15A1 Protein, HA-tagged

• Cat.No.: COL15A1-06H
• Product Overview: Recombinant human COL15A1 (Asn1133-Ser1381) protein with a Hemagglutinin Tag (YPYDVPDYA) was expressed in HEK293 cell.

Specification

• Description: This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration.
• Source: HEK293
• Species: Human
• Tag: HA
• Form: Noncovalently-linked homotrimer
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Collagen XV alpha 1/COL15A1, immobilized at 0.5 μg/mL, is able to significantly enhance neurite outgrowth.
• Molecular Mass: 29 kDa
• N-terminal Sequence Analysis: Tyr
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: COL15A1 collagen type XV alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL15A1
• Synonyms: COL15A1; collagen type XV alpha 1 chain; collagen alpha-1(XV) chain; collagen XV, alpha-1 polypeptide; collagen type XV proteoglycan; collagen, type XV, alpha 1; endostatin-XV; restin
• Gene ID: 1306
• mRNA Refseq: NM_001855
• Protein Refseq: NP_001846
• MIM: 120325
• UniProt ID: P39059