Active Recombinant Human CSF2 Protein (Ala18-Glu144), GMP Grade, Animal-Free
Cat.No. : | CSF2-31HG |
Product Overview : | GMP recombinant human GM-CSF protein (Ala18-Glu144) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines. |
- Specification
- Gene Information
- Related Products
Description : | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. |
Source : | E. coli |
Species : | Human |
Bio-activity : | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/mL. The specific activity of recombinant human GM-CSF is >1.0 x 107 IU/mg, which is calibrated against the human GM-CSF WHO International Standard. |
Molecular Mass : | Predicted Molecular Mass: 14.5 kDa SDS-PAGE: 14 kDa, reducing conditions |
Protein Length : | Ala18-Glu144 |
N-terminal Sequence Analysis : | Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr |
Endotoxin : | <0.10 EU/ μg of the protein by the LAL method. |
Purity : | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, ≤ -20 centigrade under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 100-200 μg/mL in PBS. |
Shipping : | The product is shipped with polar packs. |
Gene Name : | CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ] |
Official Symbol : | CSF2 |
Synonyms : | CSF2; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; GM CSF; GMCSF; granulocyte macrophage colony stimulating factor; molgramostin; sargramostim; CSF; colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; |
Gene ID : | 1437 |
mRNA Refseq : | NM_000758 |
Protein Refseq : | NP_000749 |
MIM : | 138960 |
UniProt ID : | P04141 |
Products Types
◆ Recombinant Protein | ||
Csf2-012C | Active Recombinant Rat Csf2 Protein (128 aa) | +Inquiry |
CSF2-492H | Recombinant Human CSF2 Protein, Biotinylated | +Inquiry |
CSF2-50P | Recombinant Active Pig CSF2 Protein, His-tagged(N-ter) | +Inquiry |
CSF2-2539H | Recombinant Human CSF2 protein(18-144 aa), N-SUMO & C-His-tagged | +Inquiry |
CSF2-43C | Active Recombinant Canine CSF2 Protein | +Inquiry |
◆ Lysates | ||
CSF2-1008MCL | Recombinant Mouse CSF2 cell lysate | +Inquiry |
CSF2-3008HCL | Recombinant Human CSF2 cell lysate | +Inquiry |
CSF2-740RCL | Recombinant Rat CSF2 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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