Active Recombinant Human CTLA4 Protein, His-Tagged

• Cat.No.: CTLA4-01H
• Product Overview: Recombinant Human CTLA4 Protein, His-Tagged was expressed in Baculovirus cell

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
• Form: Liquid
• Bio-activity: The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range ≤150ng/ml with Human B7 1/CD80.
• Molecular Mass: 40.8 kDa (368aa)
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by absorbance at 280nm)
• Storage Buffer: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte associated protein 4 [ Homo sapiens (human) ]
• Official Symbol: CTLA4
• Synonyms: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA4, ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, ATGP3364
• Gene ID: 1493
• mRNA Refseq: NM_001037631.3
• Protein Refseq: NP_001032720.1
• MIM: 123890
• UniProt ID: P16410