Active Recombinant Human CTLA4 protein, Twin Strep-tagged

• Cat.No.: CTLA4-2235H
• Product Overview: Recombinant Human CTLA4(Ala 37 - Phe 162) fused with Twin Strep tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Twin Strep
• Protein Length: Ala 37 - Phe 162
• Predicted N Terminal: Ala 37
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD80 /B7-1 Protein, With C-Fc Tag at 2 μg/mL ( 100 μl/well ) can bind Recombinant Human CTLA4 /CD152 Protein, With C-Twin Strep Tag with a linear range of 1-20 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-2 / CD86 Protein, With C-Fc Tag at 2 μg/mL ( 100 μl/well ) can bind Recombinant Human CTLA4 /CD152 Protein, With C-Twin Strep Tag with a linear range of 1-20 ng/mL.
• Molecular Mass: Has a calculated MW of 16.5 kDa. DTT-reduced Protein migrates as 21-26 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the rh CTLA4 /CD152,twin strep tag by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]
• Official Symbol: CTLA4
• Synonyms: CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3
• Gene ID: 1493
• mRNA Refseq: NM_005214
• Protein Refseq: NP_005205
• MIM: 123890
• UniProt ID: P16410
• Chromosome Location: 2q33
• Pathway: Adaptive Immune System, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CTLA4 inhibitory signaling, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem
• Function: protein binding