Active Recombinant Human CXCL12 Protein (Val24-Asn88), C-His tagged, Animal-free, Carrier-free

• Cat.No.: CXCL12-273H
• Product Overview: Recombinant Human CXCL12 Protein (Val24-Asn88) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: C-X-C motif chemokine 12 (CXCL12) also named stromal cell-derived factor 1 (SDF-1), which is a chemokine of the intercrine alpha family. CXCL12 is a 19.5kDa protein containing 167 amino acid residues. The function of CXCL12 include the hematopoiesis, angiogenesis embryogenesis, inflammation and angiogenesis. CXCL12 activated by the IL-1, TNF or lipopolysaccharide and induce the immune response in leukocytes.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4. The ED50 for this effect is < 0.5 ng/mL.
• Molecular Mass: The protein has a calculated MW of 8.55 kDa.
  The protein migrates as 10 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Val24-Asn88
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.1 M NaCl, pH 4.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: CXCL12 chemokine (C-X-C motif) ligand 12 [ Homo sapiens (human) ]
• Official Symbol: CXCL12
• Synonyms: CXCL12; chemokine (C-X-C motif) ligand 12; SDF1, SDF1A, SDF1B, stromal cell derived factor 1; stromal cell-derived factor 1; PBSF; SCYB12; SDF 1a; SDF 1b; TLSF a; TLSF b; TPAR1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; IRH; SDF1; TLSF; SDF1A; SDF1B
• Gene ID: 6387
• mRNA Refseq: NM_000609
• Protein Refseq: NP_000600
• MIM: 600835
• UniProt ID: P48061