Active Recombinant Human CXCL13 Protein (Val23-Arg94), N-His tagged, Animal-free, Carrier-free

• Cat.No.: CXCL13-08H
• Product Overview: Recombinant Human CXCL13 Protein (Val23-Arg94) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: C-X-C motif chemokine 13 (CXCL13) also named B lymphocyte chemoattractant (BLC), which is a chemokine of the intercrine alpha family. CXCL13 is a 7.9kDa protein containing 72 amino acid residues. CXCL13 is a chemotaxis for B lymphocyte. CXCL13 induces the cell proliferation though the AKT signal pathway which plays a key role intestinal cancer model. CXCL13 /CXCR5 axis is highly existed in gut, spleen and lymph nodes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to chemoattract BaF3 cells transfected with human CXCR5. The ED50 for this effect is < 20 ng/mL.
• Molecular Mass: The protein has a calculated MW of 9.49 kDa.
  The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Val23-Arg94
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: CXCL13 chemokine (C-X-C motif) ligand 13 [ Homo sapiens (human) ]
• Official Symbol: CXCL13
• Synonyms: CXCL13; chemokine (C-X-C motif) ligand 13; SCYB13, small inducible cytokine B subfamily (Cys X Cys motif), member 13 (B cell chemoattractant); C-X-C motif chemokine 13; ANGIE; ANGIE2; B cell chemoattractant; BCA 1; BLC; BLR1L; CXC chemokine BLC; B-cell chemoattractant; B-lymphocyte chemoattractant; b lymphocyte chemoattractant; small-inducible cytokine B13; B-cell-attracting chemokine 1; b cell-attracting chemokine 1; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); B-cell-homing chemokine (ligand for Burkitts lymphoma receptor-1); small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); BCA1; BCA-1; SCYB13
• Gene ID: 10563
• mRNA Refseq: NM_006419
• Protein Refseq: NP_006410
• MIM: 605149
• UniProt ID: O43927

Reviews

07/10/2021

Stability of CXCL13 was very good during long-term storage, and its properties remained unchanged after prolonged storage, indicating its excellent storage stability.

06/21/2021

The broad storage conditions of proteins make the experimental process more stable and reliable, which provides strong support for scientific research.

08/14/2019

In the process of CXCL13 production, it can be seen that it pays great attention to purity and biological activity, which makes its product extremely reliable.

Q&As

Is the expression pattern of CXCL13 protein related to the clinical prognosis of specific diseases?

Expression pattern of CXCL13 protein may be related to the clinical prognosis of specific diseases, but the specific association needs further research and verification.

What are the expression characteristics of CXCL13 protein in immune cells?

The protein is mainly expressed in immune cells by activated dendritic cells, macrophages and inflammatory cells.

Is there a regulatory mechanism for CXCL13 protein expression during inflammation?

The expression of CXCL13 protein is regulated by a variety of signaling pathways, including NF-κB, AP-1, etc. Stimulation of inflammatory factors and cytokines may lead to upregulation of CXCL13 expression.

What is the role of CXCL13 protein in lymph node metastasis?

In lymph node metastasis, the CXCL13 protein promotes the localization and metastasis of cancer cells by attracting CXCR5-positive cancer cells or immune cells.

What are the implications of CXCL13 protein research for new drug development?

CXCL13 protein plays an important role in immune regulation and inflammatory response, and studying the function and regulatory mechanism of CXCL13 protein will help the development of new drugs, such as targeted drugs targeting CXCL13 and its related signaling pathways, which may be applied to the treatment of related diseases.

What are the potential applications of CXCL13 protein in immunotherapy?

The CXCL13 protein is involved in immunomodulation, and its bound antibodies or specific receptor antagonists may contribute to the design and development of immunotherapy.