Active Recombinant Human DCC protein, His-tagged

• Cat.No.: DCC-2118H
• Product Overview: Recombinant Human DCC protein(Phe 32 - Asn 1097), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Human Netrin-1 Protein immobilized on CM5 Chip can bind Human Netrin receptor DCC, His Tag with an affinity constant of 31.8 nM as determined in a SPR assay (Biacore T200).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 119.6 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Phe 32 - Asn 1097
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DCC deleted in colorectal carcinoma [ Homo sapiens ]
• Official Symbol: DCC
• Synonyms: DCC; deleted in colorectal carcinoma; netrin receptor DCC; IGDCC1; immunoglobulin superfamily; DCC subclass; member 1; colorectal tumor suppressor; colorectal cancer suppressor; tumor suppressor protein DCC; deleted in colorectal cancer protein; immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; CRC18; CRCR1
• Gene ID: 1630
• mRNA Refseq: NM_005215
• Protein Refseq: NP_005206
• MIM: 120470
• UniProt ID: P43146