Active Recombinant Human DEFB2 protein

• Cat.No.: DEFB2-21H
• Product Overview: Active Recombinant Human DEFB2 protein(O15263)(24-64 aa) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 24-64 aa
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/ml.
• Molecular Mass: Approximately 4.3 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
• AASequence: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P
• Endotoxin: Less than 1 EU/µg of rHuBD-2 as determined by LAL method.
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.