Active Recombinant Human DKK1

• Cat.No.: DKK1-1068H
• Product Overview: Recombinant Human DKK1 (Accession # O94907) Thr32-His266, fused with , was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Non
• Protein Length: Thr32-His266
• Predicted N Terminal: Thr32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.03-0.15 µg/mL in the presence of 5 ng/ml of Recombinant Mouse Wnt-3a.
• Molecular Mass: Recombinant Human DKK1 has a calculated MW of 25.8 kDa. In SDS-PAGE migrates as 33-38 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907
• Chromosome Location: 10q11.2