Active Recombinant Human EGF protein, hFc-tagged

• Cat.No.: EGF-551H
• Product Overview: Active Recombinant Human EGF protein(P01133-1)(Asn971-Arg1023) is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 971-1023 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human EGFR, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4
• Gene ID: 1950
• mRNA Refseq: NM_001178130
• Protein Refseq: NP_001171601
• MIM: 131530
• UniProt ID: P01133