Active Recombinant Human EGFR Protein, His-GST-tagged, Alexa Fluor 488 conjugated

• Cat.No.: EGFR-31HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human EGFR (NP_005219) cytoplasmic domain (Met 668-Ala 1210), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 780
• Form: Liquid
• Bio-activity: The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
• Molecular Mass: 89.1 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU/ μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -20 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Shipping: Shipped out with blue ice.
• Conjugation: Alexa Fluor 488

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens (human) ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; EC 2.7.10.1; ERBB; ERBB1; HER1; PIG61; mENA; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); EC 2.7.10; Proto-oncogene c-ErbB-1; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663; OTTHUMP00000209210; OTTHUMP00000209211
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533