Active Recombinant Human ENPP3 protein, His-tagged, Low Endotoxin

• Cat.No.: ENPP3-1017H
• Product Overview: Recombinant Human ENPP3 protein(O14638)(Leu48-Ile875), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 48-875 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ENPP-3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 96.15 kDa. Due to glycosylation, the protein migrates to 130-160 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ENPP3 ectonucleotide pyrophosphatase/phosphodiesterase 3 [ Homo sapiens ]
• Official Symbol: ENPP3
• Synonyms: ENPP3; ectonucleotide pyrophosphatase/phosphodiesterase 3; PDNP3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; B10; CD203c; gp130RB13 6; PD IBETA; E-NPP 3; gp130RB13-6; phosphodiesterase I beta; phosphodiesterase-I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); NPP3; PD-IBETA
• Gene ID: 5169
• mRNA Refseq: NM_005021
• Protein Refseq: NP_005012
• MIM: 602182
• UniProt ID: O14638