Active Recombinant Human EPCAM Protein, Fc-tagged, Alexa Fluor 488 conjugated
Cat.No. : | EPCAM-475HAF488 |
Product Overview : | Alexa Fluor 488 conjugated recombinant human EPCAM(Gln24 - Lys265) fused with Fc tag at C-terminal was expressed in NS0. |
Availability | February 08, 2025 |
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Species : | Human |
Source : | Mammalian Cells |
Tag : | Fc |
ProteinLength : | Gln24-Lys265 |
Form : | Lyophilized |
Bio-activity : | Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.When 5 × 10^4 cells/well are added to recombinant human EpCAM/Fc Chimera and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is typically 0.7-2.8 μg/mL. Measured by its binding ability in a functional ELISA. The activity of this protein is measured in a homophilic binding assay. |
Molecular Mass : | Predicted Molecular Mass: 54 (monomer) kDa; SDS-PAGE: 60-70 kDa, reducing conditions |
N-terminal Sequence Analysis : | Gln 24 |
Endotoxin : | < 0.1 EU/ μg of the protein by the LAL method. |
Purity : | > 90 % by SDS-PAGE and analyzed by silver stain |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 488 via amines Excitation Wavelength: 488 nm Emission Wavelength: 515-545 nm |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Concentration : | 100 μg/mL |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Gene Name | EPCAM epithelial cell adhesion molecule [ Homo sapiens ] |
Official Symbol | EPCAM |
Synonyms | EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2; |
Gene ID | 4072 |
mRNA Refseq | NM_002354 |
Protein Refseq | NP_002345 |
MIM | 185535 |
UniProt ID | P16422 |
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Not For Human Consumption!
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