Active Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated

• Cat.No.: FCGR1A-373H
• Product Overview: Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # P12314-1). Predicted N-terminus: Gln 16. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 34.3 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln 16 - Pro 288
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Yervoy Ipilumimab at 5 μg/mL (100 μL/well) can bind Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated with a linear range of 0.2-3 ng/mL (QC tested).
  Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 1.02 nM as determined in a SPR assay (Biacore T200) (QC tested).
• Molecular Mass: 34.3 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
• Official Symbol: FCGR1A
• Synonyms: FCGR1A; CD64; CD64A; Fc-gamma RI; fc-gamma RIA; FCRI; IGFR1; FLJ18345
• Gene ID: 2209
• mRNA Refseq: NM_000566
• Protein Refseq: NP_000557
• MIM: 146760
• UniProt ID: P12314