Active Recombinant Human FGF1 Protein (Phe16-Asp155), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF1-93H
• Product Overview: Recombinant Human FGF1 Protein (Phe16-Asp155) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors-1 (FGF-1) is a Growth Factors of mitogenic peptides which is a 15 kDa protein containing 139 amino acid residues. Fibroblast Growth Factors-1 is secreted by the macrophage which induce endothelial cell proliferation and angiogenesis. During wound injury, fibroblast Growth Factors-1 helps the tissue remodeling. When the fibroblast Growth Factors-1 binds with the FGFR on the cell membrane which is endocytosed by FGFR and induces the cell cycle.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 16.77 kDa.
  The protein migrates as 18kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Phe16-Asp155
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF1 fibroblast growth factor 1 (acidic) [ Homo sapiens (human) ]
• Official Symbol: FGF1
• Synonyms: FGF1; fibroblast growth factor 1 (acidic); FGFA; fibroblast growth factor 1; AFGF; ECGF; ECGF beta; ECGFA; ECGFB; endothelial cell growth factor; alpha; beta; FGF alpha; GLIO703; HBGF1; heparin binding growth factor 1; heparin-binding growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha; FGF-1; HBGF-1; ECGF-beta; FGF-alpha
• Gene ID: 2246
• mRNA Refseq: NM_000800
• Protein Refseq: NP_000791
• MIM: 131220
• UniProt ID: P05230