Active Recombinant Human FGF4 Protein (Gly25-Leu206), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF4-96H
• Product Overview: Recombinant Human FGF4 Protein (Gly25-Leu206) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors-4 (FGF-4) is a 22 kDa member of the fibroblast Growth Factors with 206 amino acid residues. FGF-4 can regulate embryonic development, cell proliferation, and cell differentiation. FGF-4 is an important role development during embryogenesis.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 2.5 ng/mL. The specific activity of recombinant human FGF-4 is > 4 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 20.70 kDa.
  The protein migrates as 22 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gly25-Leu206
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS containing 0.1% sarkosyl, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF4 fibroblast growth factor 4 [ Homo sapiens (human) ]
• Official Symbol: FGF4
• Synonyms: FGF4; fibroblast growth factor 4; heparin secretory transforming protein 1 , HSTF1; HBGF 4; HST; HST 1; human stomach cancer; transforming factor from FGF related oncogene; K FGF; kaposi sarcoma oncogene; KFGF; transforming protein KS3; FGF-4; HSTF-1; oncogene HST; heparin-binding growth factor 4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; fibroblast growth factor 4 splice isoform; human stomach cancer, transforming factor from FGF-related oncogene; HST-1; HSTF1; K-FGF; HBGF-4
• Gene ID: 2249
• mRNA Refseq: NM_002007
• Protein Refseq: NP_001998
• MIM: 164980
• UniProt ID: P08620