Active Recombinant Human FH protein, His-tagged

• Cat.No.: FH-1175H
• Product Overview: Recombinant Human FH protein(Ala45-Lys510)(P07954-1), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 10mM Tris, 5mM EDTA, 1mM DTT, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to transform 1umole of Fumarate to L-malate per minute at pH 7.5 at 37°C. Specific activity is > 25 unit/mg.
• Molecular Mass: The recombinant human FH consisting of 477 amino acids and has a calculated molecular mass of 52 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Ala45-Lys510
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FH fumarate hydratase [ Homo sapiens ]
• Official Symbol: FH
• Synonyms: FH; fumarate hydratase; fumarate hydratase, mitochondrial; fumarase; MCL; LRCC; HLRCC; MCUL1
• Gene ID: 2271
• mRNA Refseq: NM_000143
• Protein Refseq: NP_000134
• MIM: 136850
• UniProt ID: P07954